Coding
Part:BBa_K3619006:Design
Designed by: Isak Ingerholt Group: iGEM20_Lund (2020-09-13)
cDNA encoding the ani microbial peptide EAFP1.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence is codon optimized for expression in E. coli K12.
Source
The protein originates from the bark of Eucommia ulmoides, rubber tree. DNA sequence was obtained though back translation.
References
1. uniprot.org/uniprot/P83596